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Vimentin anticorps (AA 63-90)

Name: Vimentin anticorps (AA 63-90)
SKU: ABIN389211
Price: 475.5 EUR
Availability: InStock

VIM Reactivité: Humain, Souris WB, IF, IHC (p) Hôte: Lapin Polyclonal RB15215 unconjugated

All lanes : Anti-Vimentin Antibody (S82) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. (Vimentin anticorps (AA 63-90))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) (ABIN389211 and ABIN2839366) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Vimentin anticorps (AA 63-90))

IHC (p)

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0. (Vimentin anticorps (AA 63-90))

Western blot analysis of Vimentin-S82 (ABIN389211 and ABIN2839366) in Hela cell line lysates (35 μg/lane). (Vimentin anticorps (AA 63-90))

Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (ABIN389211 and ABIN2839366). (Vimentin anticorps (AA 63-90))

5 images

N° du produit ABIN389211

Fiche de données

Antigène Voir toutes Vimentin (VIM) Anticorps

Vimentin (VIM)
Épitope
32

18

16

16

16

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 63-90
Reactivité
333

127

125

61

59

44

43

37

30

22

20

14

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Humain, Souris
Hôte
189

130

9

6

4

4

2
Lapin
Anticorps secondaires appropriés
Clonalité
197

146
Polyclonal
Conjugué
200

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Cet anticorp Vimentin est non-conjugé
Application
282

132

99

99

96

64

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Homologie

B, Ha, Pr, Rat, Sh

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.

Clone

RB15215

Isotype

Ig Fraction

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anti-Vimentin (VIM) (AA 2-466) antibody
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Indications d'application

IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Antigène

Vimentin (VIM)

Autre désignation

Vimentin (VIM Produits)

Synonymes

anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S

Sujet

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Poids moléculaire

53652

ID gène

7431

NCBI Accession

NP_003371

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis