CRY1 anticorps (Cryptochrome 1 (Photolyase-Like)) (AA 556-586)

Details for Product anti-CRY1 Antibody No. ABIN390079, Fournisseur: Connectez-vous pour afficher
Antigène
  • cry1-A
  • CRY1
  • cry2
  • phll1
  • xCRY1
  • PHLL1
  • AU020726
  • AU021000
  • Phll1
  • ATCRY1
  • BLU1
  • BLUE LIGHT UNINHIBITED 1
  • CRYPTOCHROME 1 APOPROTEIN (BLUE LIGHT PHOTORECEPTOR
  • ELONGATED HYPOCOTYL 4
  • HY4
  • OOP2
  • OUT OF PHASE 2
  • T3H13.14
  • T3H13_14
  • cryptochrome 1
  • cryptochrome circadian clock 1 L homeolog
  • cryptochrome circadian regulator 1
  • cryptochrome circadian clock 1
  • Cryptochrome-1
  • cryptochrome 1
  • cryptochrome 1 (photolyase-like)
  • cry1.L
  • CRY1
  • cry1
  • siu50817b
  • Cry1
Épitope
AA 556-586, C-Term
16
10
6
4
4
2
2
2
2
1
1
1
1
1
Reactivité
Humain
77
27
14
3
2
2
2
2
2
1
1
1
1
1
Hôte
Lapin
66
18
2
Clonalité (Clone)
Polyclonal ()
Conjugué
Cet anticorp CRY1 est non-conjugé
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
84
46
40
14
10
6
4
2
2
1
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Immunogène This Cry1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 556-586 AA from the C-terminal region of human Cry1.
Clone RB1844
Isotype Ig Fraction
Specificité This Cry1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557~586 amino acids from the C-terminal region of human Cry1.
Homologie Monkey
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Plasmids, Primers & others Plasmids, Primers & others CRY1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Cry1 (CRY1 Antibody Extrait)
Sujet Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central モgears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function, in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
Synonyms: Cryptochrome-1, PHLL1
Poids moléculaire 66394 DA
ID gène 1407
UniProt Q16526
Pathways Response to Water Deprivation, Proton Transport
Indications d'application WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption 6 months
Images (Fournisseur)
Western Blotting (WB) image for anti-Cryptochrome 1 (Photolyase-Like) (CRY1) (AA 556-586), (C-Term) antibody (ABIN390079) The anti-Cry1 N-term Pab (ABIN390079) is used in Western blot to detect Cry1 in place...
Immunohistochemistry (IHC) image for anti-Cryptochrome 1 (Photolyase-Like) (CRY1) (AA 556-586), (C-Term) antibody (ABIN390079) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Produit citée dans: Wang, Wang, Liu, Liu, Tay, Walsh, Yang, Wu: "CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins." dans: Insect biochemistry and molecular biology, Vol. 87, pp. 147-153, 2017 (PubMed). Méthode utilisée: Western Blotting (WB)

Background publications Griffin, Staknis, Weitz: "Light-independent role of CRY1 and CRY2 in the mammalian circadian clock." dans: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 768-71, 1999 (PubMed).

van der Horst, Muijtjens, Kobayashi, Takano, Kanno, Takao, de Wit, Verkerk, Eker, van Leenen, Buijs, Bootsma, Hoeijmakers, Yasui: "Mammalian Cry1 and Cry2 are essential for maintenance of circadian rhythms." dans: Nature, Vol. 398, Issue 6728, pp. 627-30, 1999 (PubMed).

Kobayashi, Kanno, Smit, van der Horst, Takao, Yasui: "Characterization of photolyase/blue-light receptor homologs in mouse and human cells." dans: Nucleic acids research, Vol. 26, Issue 22, pp. 5086-92, 1999 (PubMed).

Hsu, Zhao, Zhao, Kazantsev, Wang, Todo, Wei, Sancar: "Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins." dans: Biochemistry, Vol. 35, Issue 44, pp. 13871-7, 1996 (PubMed).

Avez-vous cherché autre chose?