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GAPDH anticorps (C-Term)

Name: GAPDH anticorps (C-Term)
SKU: ABIN392354
Price: 475.5 EUR
Availability: InStock

GAPDH Reactivité: Humain WB, IF, IHC (p), FACS Hôte: Lapin Polyclonal RB16543 unconjugated

Western blot analysis of GDH Antibody (C-term ) 7873b in , , CEM cell line lysates (35 μg/lane). (GAPDH anticorps (C-Term))

Flow cytometric analysis of HepG2 cells using GDH Antibody (C-term )(bottom histogram) compared to a negative control cell (top histogram). (GAPDH anticorps (C-Term))

FACS

Confocal immunofluorescent analysis of GDH Antibody (C-term ) 7873b with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (GAPDH anticorps (C-Term))

All lanes : Anti-GDH Antibody (C-term ) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (GAPDH anticorps (C-Term))

Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GDH Antibody (C-term ) 7873b. (GAPDH anticorps (C-Term))

Formalin-fixed and paraffin-embedded human prostate carcinoma with GDH Antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (GAPDH anticorps (C-Term))

IHC (p)

7 images

(1 reference)

N° du produit ABIN392354

Fiche de données

Antigène Voir toutes GAPDH Anticorps

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Épitope
36

21

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 233-259, C-Term
Reactivité
211

142

135

75

65

55

36

28

23

21

20

19

18

16

12

12

11

10

9

8

6

4

4

4

4

3

3

3

3

3

2

2

1

1

1

1

1
Humain
Hôte
158

97

17

10

4
Lapin
Anticorps secondaires appropriés
Clonalité
179

105
Polyclonal
Conjugué
155

35

22

16

5

5

5

5

4

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1
Cet anticorp GAPDH est non-conjugé
Application
233

98

72

70

45

40

35

30

16

16

15

15

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Homologie

C, M, Pig, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène

This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.

Clone

RB16543

Isotype

Ig Fraction

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GAPDH Reactivité: Humain WB, IF, IHC (p), IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH Reactivité: Humain, Souris, Rat, Lapin, Porc, Poulet, Boeuf (Vache), Différentes espèces WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH Reactivité: Humain WB, IHC, IF, IP Hôte: Lapin Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivité: Humain, Souris, Rat WB, IF, IHC (p) Hôte: Souris Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reactivité: Humain, Rat WB, ELISA, IHC, IF Hôte: Chèvre Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF Hôte: Chèvre Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivité: Humain, Souris, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reactivité: Humain WB, IF, IHC (p) Hôte: Lapin Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

IF: 1:10~50. IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Wang, Chen, Wang, Yu, Zhao, Zhang, Tang, Peng: "Metalloproteases meprin-ɑ (MEP1A) is a prognostic biomarker and promotes proliferation and invasion of colorectal cancer." dans: BMC cancer, Vol. 16, pp. 383, (2017) (PubMed).
Antigène

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation

GAPDH (GAPDH Produits)

Synonymes

anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985

Sujet

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Poids moléculaire

36053

ID gène

2597

NCBI Accession

NP_001243728, NP_002037

UniProt

P04406