HBxAg anticorps

N° du produit ABIN393198

Cet anticorps anti-HBxAg Monoclonal Souris (Clone 3F6-G10) (ABIN393198) détecte spécifiquement HBxAg dans WB, ELISA, IHC (fro), IHC (p) et IHC. L’anticorps est réactif avec des échantillons de Hepatitis B Virus (HBV).

Aperçu rapide pour HBxAg anticorps (ABIN393198)

Antigène: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Reactivité: Hepatitis B Virus (HBV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HBxAg est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Clone: 3F6-G10

Détail du produit anti-HBxAg anticorps

Specificité: Recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.

Purification: Affinity purified

Immunogène: HB-Xag-Protein A Fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:100 - 1:500)

Usage: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Commentaires:

Target Species of Antibody: Hepatitis B Virus

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 0.1 % BSA.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Détails sur HBxAg

Antigène: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Autre désignation: Hepatitis B X Antigen (HBxAg)

Classe de substances: Viral Protein