CRE Recombinase (CRE) anticorps

N° du produit ABIN398566

Détail du produit

Antigène: CRE Recombinase (CRE)

Reactivité: Bacteriophage P1

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Specificité: Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.

Réactivité croisée (Details): Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.

Purification: Protein A affinity column

Immunogène: Purified recombinant Cre recombinase protein

Clone: 1D8H3

Isotype: IgG2a

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1.0 µg/mL Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Référence

Lin, Liu, Sun, Yuan, Zhang, Chen: "Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line." dans: In vitro cellular & developmental biology. Animal, Vol. 49, Issue 2, pp. 114-21, (2013) (PubMed).

Kaushik, Arias, Kwon, Lopez, Athonvarangkul, Sahu, Schwartz, Pessin, Singh: "Loss of autophagy in hypothalamic POMC neurons impairs lipolysis." dans: EMBO reports, Vol. 13, Issue 3, pp. 258-65, (2012) (PubMed).

Détail du antigène

Antigène: CRE Recombinase (CRE)

Autre désignation: Cre Recombinase (CRE Produits)

Synonymes: anticorps cyclization recombinase, anticorps cre

Classe de substances: Phage Protein

Sujet: Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of Cre recombinase in different protein expression systems.