PDCD4 anticorps (pSer457)

N° du produit ABIN400797

Cet anticorps Souris Monoclonal détecte spécifiquement PDCD4 dans WB, IHC (p), EIA et IHC (fro). Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Xenopus laevis.

Aperçu rapide pour PDCD4 anticorps (pSer457) (ABIN400797)

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDCD4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 9G6

Détail du produit anti-PDCD4 anticorps

Épitope: pSer457

Specificité: This antibody is specific for Pdcd4 protein phosphorylated at Ser457. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with Human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Xenopus (100 %).
Species reactivity (tested):Human.

Attributs du produit: Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like

Purification: Protein A Chromatography

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 μg/mL, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25μl) dilute 1: 10 by adding 225 μl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur PDCD4

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Autre désignation: PDCD4

Sujet: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

ID gène: 27250

UniProt: Q53EL6