The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
Réactivité croisée (Details)
Species reactivity (tested):Human.
Attributs du produit
Synonyms: PSGL-1, Selectin P ligand, SELPLG, P-selectin glycoprotein ligand 1
SELPLG
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow cytometry: 1.5 - 2.5 μg/mL. Positive control: human peripheral blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing. Shelf life: one year from despatch.
CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand