UBA1 anticorps

N° du produit ABIN401028

Détail du produit anti-UBA1 anticorps

Antigène: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reactivité: Humain, Rat, Souris, Chien, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against Ubiquitin Activating Enzyme E1 protein.

Réactivité croisée (Details): Species reactivity (expected):Rabbit, Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Purified

Immunogène: Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Reconstitute with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur UBA1

Antigène: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Autre désignation: UBE1 / UBA1 (UBA1 Produits)

Synonymes: anticorps A1S9, anticorps A1S9T, anticorps A1ST, anticorps AMCX1, anticorps GXP1, anticorps POC20, anticorps SMAX2, anticorps UBA1A, anticorps UBE1, anticorps UBE1X, anticorps DDBDRAFT_0190927, anticorps DDBDRAFT_0220497, anticorps DDB_0190927, anticorps DDB_0220497, anticorps Sbx, anticorps Ube-1, anticorps Ube1x, anticorps a1s9, anticorps a1s9t, anticorps a1st, anticorps amcx1, anticorps gxp1, anticorps poc20, anticorps smax2, anticorps uba1a, anticorps ube1, anticorps ube1x, anticorps uba1, anticorps uba1b, anticorps ube, anticorps zgc:66143, anticorps wu:fa01e08, anticorps wu:fb30f01, anticorps wu:fi21c11, anticorps wu:fj14g11, anticorps ubiquitin like modifier activating enzyme 1, anticorps ubiquitin activating enzyme E1, anticorps Ubiquitin-conjugating enzyme E1, anticorps ubiquitin-like modifier activating enzyme 1, anticorps ubiquitin-like modifier activating enzyme 1 S homeolog, anticorps ubiquitin-like modifier activating enzyme 1 L homeolog, anticorps UBA1, anticorps uae1, anticorps GL50803_4083, anticorps GL50803_10661, anticorps Uba1, anticorps uba1.S, anticorps uba1.L, anticorps uba1, anticorps ptr3

Sujet: Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1

ID gène: 7317

NCBI Accession: NP_003325

UniProt: P22314