UBA1 anticorps

N° du produit ABIN401028

Cet anticorps anti-UBA1 Polyclonal Lapin (ABIN401028) détecte spécifiquement UBA1 dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Chien et Lapin.

Aperçu rapide pour UBA1 anticorps (ABIN401028)

Antigène: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reactivité: Humain, Rat, Souris, Chien, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-UBA1 anticorps

Specificité: This antibody is directed against Ubiquitin Activating Enzyme E1 protein.

Réactivité croisée (Details): Species reactivity (expected):Rabbit, Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Purified

Immunogène: Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Reconstitute with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur UBA1

Antigène: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Autre désignation: UBE1 / UBA1

Sujet: Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1

ID gène: 7317

NCBI Accession: NP_003325

UniProt: P22314