Cet anticorps anti-Protein G Polyclonal Lapin conjugué à FITC (ABIN401070) détecte spécifiquement Protein G dans FACS et IF.
L’anticorps est réactif avec des échantillons de Streptococcus.
Reactivité: Streptococcus
FACS, FM
Hôte: Lapin
Polyclonal
Texas Red (TR)
Indications d'application
Immunofluorescence: 1/500 - 1/2500. Flow Cytometry: 1/2000 - 1/10000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
10.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % Thimerosal as preservative
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Antigène
Protein G
Sujet
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.