L1/ORF2 anticorps

N° du produit ABIN401096

L’anticorps anti-L1/ORF2 Polyclonal Poulet est utilisé pour la détection de L1/ORF2 dans des échantillons de Chimpanzé et Humain. Il a été validé pour EIA et WB.

Aperçu rapide pour L1/ORF2 anticorps (ABIN401096)

Antigène: L1/ORF2

Reactivité: Chimpanzé, Humain

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Détail du produit

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.

Purification: IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer.

Immunogène: Two Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/10000. Western Blot: 1/2000 - 1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détail du antigène

Antigène: L1/ORF2

Autre désignation: L1 / ORF2

Sujet: The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.Synonyms: LINE-1