FITC anticorps

N° du produit ABIN401128

Cet anticorps Chèvre Polyclonal détecte spécifiquement FITC dans WB, IHC (fro) et EIA.

Aperçu rapide pour FITC anticorps (ABIN401128)

Antigène: FITC (Fluorescein Isothiocyanate (FITC))

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FITC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-FITC anticorps

Purification: Immunoaffinity Chromatography.

Immunogène: Fluorescein conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/5,000-1/30,000. Western Blot: 1/500-1/3,000. Immunohistochemistry: 1/250-1/1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur FITC

Antigène: FITC (Fluorescein Isothiocyanate (FITC))

Autre désignation: FITC

Classe de substances: Chemical

Sujet: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate