L’anticorps anti-Transferrin Polyclonal Lapin est utilisé pour la détection de Transferrin dans des échantillons de Souris. Il a été validé pour WB, EIA et IHC (fro).
Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 µm filtered, aseptically filled, and stored at -20° C. An aliquot was submitted for final qualification.
TF
Reactivité: Boeuf (Vache)
WB, IHC, ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 1,000 - 1: 5,000. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.