HA-Tag anticorps

N° du produit ABIN401240

L’anticorps Lapin Polyclonal anti-HA-Tag a été validé pour WB, EIA et IHC (p). Il convient pour détecter HA-Tag dans des échantillons de .

Aperçu rapide pour HA-Tag anticorps (ABIN401240)

Antigène: HA-Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HA-Tag est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HA-Tag anticorps

Séquence: YPYDVPDYA

Purification: Affinity Chromatography

Immunogène: Whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from Hemagglutinin Influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.

Isotype: IgG

Information d'application

Indications d'application: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. Theantibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targetedproteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and HA containing recombinant proteins. This antibody is likely functional forImmunoprecipitation, Immunocytochemistry, and other Immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels inassays and low cross-reactivity with other cellular proteins. Recommended Dilutions: ELISA: 1/20,000-1/150,000.

Restrictions: For Research Use only

Stockage

Concentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur HA-Tag

Antigène: HA-Tag

Autre désignation: HA Epitope Tag (YPYDVPDYA)

Classe de substances: Tag

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.