ATAD5 anticorps (AA 63-76)
Aperçu rapide pour ATAD5 anticorps (AA 63-76) (ABIN401276)
Antigène
Voir toutes ATAD5 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 63-76
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Specificité
- This antibody is directed against Elg1 protein.
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Réactivité croisée (Details)
- Species reactivity (tested):Human.
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Purification
- Immunoaffinity chromatography
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Immunogène
- Synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
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Isotype
- IgG
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Indications d'application
- ELISA: 1: 3,000 - 1: 15,000. Western blot: 1: 500 - 1: 2,000 (Expect a band approximately 120 kDa in size correspondingto Elg1 in the appropriate cell lysate or extract).
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Restrictions
- For Research Use only
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Concentration
- 0.50 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))
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Autre désignation
- ATAD5
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Sujet
- ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.Synonyms: ATPase family AAA domain-containing protein 5, C17orf41, Chromosome fragility-associated gene 1 protein, FRAG1
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ID gène
- 79915
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NCBI Accession
- NP_079133
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UniProt
- Q96QE3
Antigène
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