TUBA1B anticorps (AA 427-441)

N° du produit ABIN401310

Cet anticorps anti-TUBA1B est un anticorps Lapin Polyclonal détectant TUBA1B dans WB et EIA. Adapté pour Humain, Avian et Mammifères.

Aperçu rapide pour TUBA1B anticorps (AA 427-441) (ABIN401310)

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivité: Humain, Avian, Mammifères

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TUBA1B est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-TUBA1B anticorps

Épitope: AA 427-441

Specificité: This antibody is directed against alpha Tubulin protein. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent Loading Control.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/5,000-1/25,000. Immunofluorescence: 1/500-1/2,000Western Blot: 1/500-1/3,000. Expect a band at approx. 50 kDa in size corresponding to alpha tubulin by Western blotting inmost cell lysates or extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.08 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur TUBA1B

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Autre désignation: alpha Tubulin / TUBA1B

Sujet: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

ID gène: 10376

NCBI Accession: NP_006073

UniProt: P68363

Pathways: Dynamique des Microtubules, M Phase