NOXO1 anticorps (AA 238-252)

N° du produit ABIN401322

Cité dans 1 publication. Cet anticorps anti-NOXO1 Polyclonal Lapin (ABIN401322) détecte spécifiquement NOXO1 dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain, Rat, Chimpanzé, Chien et Cobaye.

Aperçu rapide pour NOXO1 anticorps (AA 238-252) (ABIN401322)

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reactivité: Humain, Rat, Chimpanzé, Chien, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOXO1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-NOXO1 anticorps

Épitope: AA 238-252

Specificité: This antibody is directed against NOXO1 protein. Expect a band approximately 50 kDa in size corresponding to NOXO1 protein by western blotting in the appropriate cell lysate or extract. A BLAST analysis was used to suggest cross reactivity with NOXO1 protein from Human and Chimpanzee (100 % homology). Expect reactivity with alpha, delta and gamma isoforms of NOXO1. Also expect partial reactivity against NOXO1 homologues from Dog and Guinea Pig (87 %), as well as Rat (75 %) and Mouse (68 %). Reactivity against homologues from other sources is not known.

Purification: Immunoaffinity Chromatography.

Immunogène: Recombinant protein corresponding to amino acids 238-252 of Human NOXO1 protein

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody is tested for use in ELISA, Immunohistochemistry andWestern blot. Recommended Dilutions: ELISA: 1/2,0000-1/100,000.

Restrictions: For Research Use only

Stockage

Concentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7. containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.

Références pour anticorps anti-NOXO1 (ABIN401322)

Madrigal-Matute, Fernandez-Garcia, Gomez-Guerrero, Lopez-Franco, Muñoz-Garcia, Egido, Blanco-Colio, Martin-Ventura: "HSP90 inhibition by 17-DMAG attenuates oxidative stress in experimental atherosclerosis." dans: Cardiovascular research, Vol. 95, Issue 1, pp. 116-23, (2012) (PubMed).

Détails sur NOXO1

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Autre désignation: NOXO1

Sujet: The enzymes NADPH oxidase (NOX) and dual oxidase (DUOX) generate ROS in a regulated manner, producing reactive oxygen in various cells and tissues in response to growth factors, cytokines and calcium signals. The oxidase consists of the catalytic subunit gp91phox (otherwise known as NOX2), together with the regulatory subunits p22phox, p47phox, p40phox, p67phox and the small GTPase RAC. The enzyme activity of gp91phox is regulated by the assembly of these regulatory subunits with gp91phox to form an active complex. In 1999, the first of the NOX homologues of gp91phox was described as NOX1. The enzyme was cloned from a colon epithelial cell complementary DNA library. When expressed in cells, NOX1 generated low amounts of ROS, but high-level ROS production by NOX1 was subsequently achieved by co-expression with novel regulatory subunits (described later). Subsequently, NOX3 and NOX4 were cloned, and the latter was shown to generate high levels of ROS when expressed in cells. NOX organizer 1 (NOXO1) is a homologue of p47phox and has an almost identical domain organization, except that it lacks the auto-inhibitory region. NOX activator 1 (NOXA1) is a homologue of p67phox and similarly shares the same domain organization, except that it lacks one of the two SH3 domains that are present in p67phox. Co-transfection of NOX1, NOXO1 and NOXA1 results in marked ROS generation. Similar to p47phox, NOXO1 binds to p22phox, which is required for NOX1-dependent activity. NOXA1 has a well-conserved activation domain, implying a conserved mechanism for regulating the activity of the target NOX enzyme.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5

ID gène: 124056

NCBI Accession: NP_653204

UniProt: Q8NFA2