PDCD4 anticorps (pSer457, Ser457)

N° du produit ABIN401369

L’anticorps anti-PDCD4 Polyclonal Lapin est utilisé pour la détection de PDCD4 dans des échantillons de Humain, Souris, Rat et Xenopus laevis. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour PDCD4 anticorps (pSer457, Ser457) (ABIN401369)

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDCD4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PDCD4 anticorps

Épitope: pSer457, Ser457

Specificité: This antibody is specific for Pdcd4 protein phosphorylated at Ser457.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Xenopus.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/400,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry: 21.25 - 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur PDCD4

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Autre désignation: PDCD4

Sujet: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

ID gène: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6