EIF3E anticorps (C-Term)

N° du produit ABIN401422

Cet anticorps Lapin Polyclonal détecte spécifiquement EIF3E dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Singe.

Aperçu rapide pour EIF3E anticorps (C-Term) (ABIN401422)

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF3E est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-EIF3E anticorps

Épitope: C-Term

Specificité: This antibody is specific for Mouse eIF3S6/Int6 protein.

Réactivité croisée (Details): Species reactivity (expected):Human, Rat, Canine, Bovine, Monkey.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6 . Genename: EIF3E

Isotype: IgG

Information d'application

Indications d'application: Suitable for use in ELISA (1/80,000) and Western Blot (1/1,000). Expect a band approx. 48 kDa in size corresponding to eIF3S6/Int6 by Western blotting in theappropriate cell lysate or extract. This antibody is capable of detecting bothover-expressed and endogenous eIF3S6/Int6.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.8 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur EIF3E

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Autre désignation: EIF3E / EIF3S6

Sujet: Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.Synonyms: Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48

ID gène: 16341

NCBI Accession: NP_032414

UniProt: P60229

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C