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MLF1IP anticorps (N-Term)

MLF1IP Reactivité: Humain, Chimpanzé, Cheval WB, EIA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN401441
  • Antigène Voir toutes MLF1IP Anticorps
    MLF1IP (MLF1 Interacting Protein (MLF1IP))
    Épitope
    • 15
    • 8
    • 8
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 44
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain, Chimpanzé, Cheval
    Hôte
    • 44
    Lapin
    Clonalité
    • 44
    Polyclonal
    Conjugué
    • 20
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp MLF1IP est non-conjugé
    Application
    • 42
    • 23
    • 14
    • 14
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificité
    This antibody is directed against MLF1IP protein.
    Réactivité croisée (Details)
    Species reactivity (expected):Chimpanzee, Horse, Bovine, Canine, Mouse, Rat, Chicken.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Immunogène
    Recombinant protein corresponding to the amino terminal end of human MLF1IP protein
    Isotype
    IgG
    Top Product
    Discover our top product MLF1IP Anticorps primaire
  • Indications d'application
    ELISA: 1/650,000. Western Blot: 1/500- 1/2,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.64 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
  • Antigène
    MLF1IP (MLF1 Interacting Protein (MLF1IP))
    Autre désignation
    MLF1IP (MLF1IP Produits)
    Synonymes
    anticorps MGC147270, anticorps CENP50, anticorps CENPU, anticorps CENPU50, anticorps KLIP1, anticorps PBIP1, anticorps CENP-U, anticorps CENP-50, anticorps 1700029A22Rik, anticorps centromere protein U, anticorps MLF1 interacting protein, anticorps cenpu, anticorps MLF1IP, anticorps CENPU, anticorps Cenpu
    Sujet
    Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sisterchromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.Synonyms: CENP-U(50), CENPU, Centromere protein U, ICEN24, Interphase centromere complex protein 24, KLIP1, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, PBIP, Polo-box-interacting protein 1
    ID gène
    79682
    NCBI Accession
    NP_078905
    UniProt
    Q71F23
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