TNKS anticorps (N-Term)

N° du produit ABIN401535

Cet anticorps anti-TNKS Polyclonal Chèvre (ABIN401535) détecte spécifiquement TNKS dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TNKS anticorps (N-Term) (ABIN401535)

Antigène: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TNKS est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-TNKS anticorps

Épitope: N-Term

Specificité: This antibody detects Tankyrase-1 (TANK1) at N-term. No cross reactivity expected to TANK2.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by affinity chromatography using Protein G

Immunogène: Fusion protein containing 120aa amino acids from N Terminal region of the protein sequence according to NP_003738.2 . Genename: TNKS

Information d'application

Indications d'application: Western blot: In transfected HEK293 transiently expressing TANK1 a band of approx.142 kDa is observed. This band is not observed in the non-transfected HEK293. Thecalculated molecular size is 142 kDa according to NP_003738.2. Recommendedconcentration, 0.5-1.5 μg/mL. Full caption )see picture below): HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4)and probed with EB02508 (mock transfection in first lane). Lane three is empty. Lower

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur TNKS

Antigène: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Autre désignation: Tankyrase-1 (TANK1)

Sujet: Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP) ribosylation. Since ADP ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP) ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase colocalizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes. Tankyrase may regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4 vesicles.Synonyms: PARP5A, PARPL, TIN1, TINF1, TNKS, TNKS-1, TNKS1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I

ID gène: 8658

NCBI Accession: NP_003738

UniProt: O95271