EIF4B anticorps (AA 420-424)

N° du produit ABIN401568

Cet anticorps anti-EIF4B Polyclonal Lapin (ABIN401568) détecte spécifiquement EIF4B dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour EIF4B anticorps (AA 420-424) (ABIN401568)

Antigène: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EIF4B anticorps

Épitope: AA 420-424

Specificité: This antibody detects endogenous levels of total eIF4B protein.

Purification: Immunoaffinity Chromatography using epitope-specific peptide.

Immunogène: Peptide sequence around amino acids 420~424 (T-G-S-E-S) derived from Human eIF4B.

Information d'application

Indications d'application: Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Détails sur EIF4B

Antigène: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Autre désignation: EIF4B

Sujet: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B

ID gène: 1975

NCBI Accession: NP_001408

UniProt: P23588