Myc Tag anticorps (Biotin)

N° du produit ABIN401744

L’anticorps anti-Myc Tag Polyclonal Lapin est utilisé pour la détection de Myc Tag. Il a été validé pour WB, IHC (fro) et EIA.

Aperçu rapide pour Myc Tag anticorps (Biotin) (ABIN401744)

Antigène: Myc Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myc Tag est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-Myc Tag anticorps (Biotin)

Séquence: EQKLISEEDL

Specificité: This antibody is directed against human c-myc.

Attributs du produit: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Affinity chromatography

Immunogène: Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Information d'application

Indications d'application: ELISA: 1/4,000 - 1/20,000. Western Blot: 1/400 - 1/2,000. Immunohistochemistry: 1/400 - 1/2,000.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent). For extended storage mix with glycerol to 50 % .

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as perservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur Myc Tag

Antigène: Myc Tag

Autre désignation: C-Myc Epitope Tag

Classe de substances: Tag

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag

ID gène: 4609

NCBI Accession: NP_002458

UniProt: P01106