GFP anticorps (Texas Red (TR))

N° du produit ABIN401757

Cet anticorps anti-GFP Monoclonal Souris conjugué à Texas Red (TR) (Clone 9F9-F9) (ABIN401757) détecte spécifiquement GFP dans WB, IF et EIA. L’anticorps est réactif avec des échantillons de Aequorea victoria.

Aperçu rapide pour GFP anticorps (Texas Red (TR)) (ABIN401757)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est conjugé à/à la Texas Red (TR)

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 9F9-F9

Détail du produit anti-GFP anticorps (Texas Red (TR))

Specificité: This antibody reacts to Green Fluorescent Protein (GFP).

Attributs du produit: Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 3.0 moles Texas Red(TM) per mole of Mouse IgG

Purification: Affinity chromatography on Protein A

Immunogène: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria

Isotype: IgG2a

Information d'application

Indications d'application: ELISA. Western Blot. Immunohistochemistry.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm where ε0.1% = 0.71)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution stable for a short term at 2-8 °C, but best stored at -20 °C. Following reconstitution store the antibody at -20 °C. Do not aliquot in less than 100 μl.

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

ID gène: 6100

UniProt: P42212