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Recombinant RFP anticorps (Atto 488)

RFP Reactivité: Discosoma IF, ICC, FACS Hôte: Alpaca Monoclonal 2B12 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
N° du produit ABIN4368319
  • Antigène Voir toutes RFP Anticorps
    RFP (Red Fluorescent Protein (RFP))
    Type d'anticorp
    Recombinant Antibody
    Fragment
    single-domain Antibody (sdAb)
    Reactivité
    • 52
    • 1
    Discosoma
    Hôte
    • 23
    • 15
    • 8
    • 5
    • 3
    Alpaca
    Expression System
    E.coli
    Clonalité
    • 31
    • 23
    Monoclonal
    Conjugué
    • 21
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RFP est conjugé à/à la Atto 488
    Application
    • 29
    • 21
    • 15
    • 11
    • 9
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Fonction
    Camelid sdAb anti-RFP conjugated with Atto488, Clone 2B12
    Specificité
    Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
    Réactivité croisée (Details)
    Does not cross-react with GFP or mTagBFP derivatives.
    Attributs du produit
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    Purification
    Produced in: E.coli
    Immunogène
    RFP
    Clone
    2B12
    Labeling Ratio
    a single fluorophore is coupled to exactly one sdAb
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  • Indications d'application
    Recommended dilution 1:500
    Commentaires

    Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    5 μM
    Buffer
    5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Protect from light!
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    Date de péremption
    12 months
  • Antigène
    RFP (Red Fluorescent Protein (RFP))
    Autre désignation
    RFP (RFP Produits)
    Poids moléculaire
    26 kDa
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