Recombinant RFP anticorps (AZDye 568)

N° du produit ABIN4368331

L’anticorps Alpaca Monoclonal anti-RFP a été validé pour IF, FACS et ICC. Il convient pour détecter RFP dans des échantillons de Discosoma.

Aperçu rapide pour Recombinant RFP anticorps (AZDye 568) (ABIN4368331)

Antigène: RFP (Red Fluorescent Protein (RFP))

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Discosoma

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp RFP est conjugé à/à la AZDye 568

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2B12

Détail du produit anti-RFP anticorps (AZDye 568)

Expression System: E.coli

Fonction: Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 2B12

Specificité: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Réactivité croisée (Details): Does not cross-react with GFP or mTagBFP derivatives.

Attributs du produit: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Immunogène: Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Information d'application

Indications d'application: Recommended dilution 1:500

Commentaires:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Conseil sur la manipulation: Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

Stock: -20 °C,-80 °C

Stockage commentaire: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Date de péremption: 12 months

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: RFP

Poids moléculaire: 26 kDa