EpCAM anticorps

N° du produit ABIN452356

Cet anticorps Souris Monoclonal détecte spécifiquement EpCAM dans WB, IHC (p) et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour EpCAM anticorps (ABIN452356)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HEA125

Détail du produit anti-EpCAM anticorps

Specificité: Clone HEA125 recognizes Ep-CAM (polypeptide reacting: Mr 40,000 epithelium-specific cell surface glycoprotein). HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. Tested Reactivities on Cultured Cell Lines: All carcinoma cell lines tested so far, particularly strong reaction with colon carcinoma cell lines (HT-29, WiDr, SW1116). Polypeptide Reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: HT-29 colon carcinoma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting. Cell sorting. Cytological Material. Immunofluorescence microscopy. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pre-treatment is required prior toantibody application. Working Dilutions: Ready-to-use for immunohistochemical application.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: CD326 / EPCAM / TACSTD1

Sujet: This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

ID gène: 4072

NCBI Accession: NP_002345

UniProt: P16422