Calretinin anticorps

N° du produit ABIN452385

Cet anticorps anti-Calretinin est un anticorps Souris Monoclonal détectant Calretinin dans WB, IHC (p) et IHC (fro). Adapté pour Humain et Rat.

Aperçu rapide pour Calretinin anticorps (ABIN452385)

Antigène: Calretinin (CALB2) (Calbindin 2 (CALB2))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calretinin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 6B3

Détail du produit anti-Calretinin anticorps

Specificité: Specific for Calretinin. No cross-reaction with Calbindin and Calmodulin. The antibody is suitable for the detection of Calretinin in neurons and may be used as an aid in differential diagnosis of pleura-mesothelioma. This marker is frequently expressed in epitheloid mesothelioma, while occasionally it turns positive in sarcomatous mesothelioma and carcinoma. It was found to be negative in sarcoma (2,3) and in 90 % of adenocarcinoma.

Réactivité croisée (Details): Species reactivity (tested):Human and Rat.

Attributs du produit: Synonyms: CALB2, CAB29, 29 kDa calbindin

Immunogène: Recombinant Human Calretinin 22kDa.

Isotype: Ig Fraction

Information d'application

Indications d'application: Immunohistochemistry on Frozen and Paraffin Sections. (Improvement of staining withUnmasking Fluid G or Unmasking Fluid C)Immunoblotting. (1/40-1/50)Positive Control: Cerebral cortex, pleural mesothelioma. Incubation Time: 60 min at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Stabilized Phosphate buffer, pH 7.3 containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Calretinin

Antigène: Calretinin (CALB2) (Calbindin 2 (CALB2))

Autre désignation: Calretinin

Sujet: Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X, they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.Synonyms: 29 kDa calbindin, CAB29, CALB2

ID gène: 794

UniProt: P22676