HCV NS3 anticorps

N° du produit ABIN452526

Cet anticorps anti-HCV NS3 est un anticorps Souris Monoclonal détectant HCV NS3 dans WB et EIA. Adapté pour Humain.

Aperçu rapide pour HCV NS3 anticorps (ABIN452526)

Antigène: HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HCV NS3 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 806

Détail du produit anti-HCV NS3 anticorps

Réactivité croisée (Details): Species reactivity (tested):Human.

Attributs du produit: Synonyms: Hepatitis C Virus Serine proteiase NS3

Purification: Affinity Chromatography on Protein G.

Immunogène: Purified Recombinant Chimeric HCV Polyprotein (555 a. a. )

Isotype: IgG2a

Information d'application

Indications d'application: ELISA. Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 806) at 0.1-1 μg/mL will allowvisualization of 100 ng/lane of both Recombinant Chimeric HCV Polyprotein andRecombinant NS-3 Protein. No reaction can be seen with Synthetic NS-3. Testing is under reducing and non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HCV NS3

Antigène: HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))

Autre désignation: HCV NS3

Classe de substances: Viral Protein

Sujet: HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins, because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.Synonyms: Hepatitis C Virus Serine proteiase NS3