L’anticorps anti-Helicobacter Pylori Monoclonal Souris est utilisé pour la détection de Helicobacter Pylori dans des échantillons de Helicobacter pylori. Il a été validé pour WB, EIA et ELISA (Capture).
ELISA: HP CagA antibody (Clone 10E9) can be used as Capture antibody. In Sandwich ELISA, anti-HP CagA antibody clone 5H10 (Cat#ABIN452544) coated wellsmatches with HRP conjugated clone 5C6, clone 3C10 and clone 3C1 to detect therecombinant antigen. In addition, this clone selectively detected cell lysate of a CagA containing HP stain whenHRP conjugated clone 3C10 was used. Western Blotting: This clone detected a 32 KD band corresponding to the molecular weightof the recombinant CagA antigen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
Buffer
0.01 M PBS, pH 7.0 without preservatives
Agent conservateur
Without preservative
Stock
-20 °C
Stockage commentaire
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
Helicobacter Pylori (H. pylori)
Autre désignation
Helicobacter Pylori
Classe de substances
Virus
Sujet
Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20 % may develop gastritis and gastric ulcer and 1-2 % may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.Synonyms: H. pylori