HSF2 anticorps

N° du produit ABIN452671

Cet anticorps Rat Monoclonal détecte spécifiquement HSF2 dans WB, IF, IP, IHC (fro), EIA et EMSA. Il présente une réactivité avec des échantillons de Différentes espèces.

Aperçu rapide pour HSF2 anticorps (ABIN452671)

Antigène: HSF2 (Heat shock factor protein 2 (HSF2))

Reactivité: Différentes espèces

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSF2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 3-00E-002

Détail du produit anti-HSF2 anticorps

Specificité: This antibody detects an ~ 69 kDa protein in unstressed cells corresponding to HSF2 on SDS-PAGE immunoblots.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig.

Attributs du produit: Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

Purification: Purified

Immunogène: Purified recombinant mouse HSF2 protein

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 4 μg/mL was sufficient for detection of HSF2 of HeLa lysate. Gel shift assay (EMSA). Immunoprecipitation. Immunflourescence. Immunohistochemistry on frozen sections. ELISA.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HSF2

Antigène: HSF2 (Heat shock factor protein 2 (HSF2))

Autre désignation: Heat Shock Factor 2 / HSF2

Sujet: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

ID gène: 15500

UniProt: P38533