TFB1M anticorps (C-Term)

N° du produit ABIN452674

Cet anticorps Chèvre Polyclonal détecte spécifiquement TFB1M dans WB et EIA. Il présente une réactivité avec des échantillons de Humain et Chien.

Aperçu rapide pour TFB1M anticorps (C-Term) (ABIN452674)

Antigène: TFB1M (Transcription Factor B1, Mitochondrial (TFB1M))

Reactivité: Humain, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TFB1M est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-TFB1M anticorps

Épitope: C-Term

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-ELKRRKSKNEEKE, from the C-Terminus of Human TFB1M (NP_057104.2).

Information d'application

Indications d'application: TFB1M antibody can be used for: Peptide ELISA: Detection limit dilution 1/64000. Western blot: 0.3-1 μg/mL. Approx 38 kDa band observed in lysates of cell line A431(calculated MW of 39.8 kDa according to NP_057104.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Canine (Dog).

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur TFB1M

Antigène: TFB1M (Transcription Factor B1, Mitochondrial (TFB1M))

Autre désignation: TFB1M

Sujet: TFB1M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity.Synonyms: CGI-75, Dimethyladenosine transferase 1 mitochondrial, Mitochondrial 12S rRNA dimethylase 1, Mitochondrial transcription factor B1, N'-adenosyl(rRNA) dimethyltransferase 1, S-adenosylmethionine-6-N', mtTFB1

ID gène: 51106

NCBI Accession: NP_057104

UniProt: Q8WVM0