EIF5A anticorps (Internal Region, N-Term)

N° du produit ABIN452675

L’anticorps anti-EIF5A Polyclonal Chèvre est utilisé pour la détection de EIF5A dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour EIF5A anticorps (Internal Region, N-Term) (ABIN452675)

Antigène: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF5A est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-EIF5A anticorps

Épitope: Internal Region, N-Term

Specificité: Recognizes eIF5A.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of Human eIF5A.

Information d'application

Indications d'application: Peptide ELISA: eIF5A Antibody Detection limit dilution 1/128000. Western blot: 1-3 μg/mL. Approx 20 kDa band observed in MOLT4 lysates (calculated MW of20.0 kDa according to NP_001137232.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur EIF5A

Antigène: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Autre désignation: EIF5A1

Sujet: Eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein, the only protein known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. eIF5A protein was previously reported to be involved in the first step of peptide bond formation in translation, however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.Synonyms: Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A-1, Rev-binding factor, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1

ID gène: 1984

NCBI Accession: NP_001137232

UniProt: P63241

Pathways: Regulation of Muscle Cell Differentiation