YDJ1 anticorps

N° du produit ABIN453946

Cet anticorps Souris Monoclonal détecte spécifiquement YDJ1 dans IP, WB et EIA. Il présente une réactivité avec des échantillons de Saccharomyces cerevisiae.

Aperçu rapide pour YDJ1 anticorps (ABIN453946)

Antigène: YDJ1 (HSP40 / YDJ1 (YDJ1))

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp YDJ1 est non-conjugé

Application: Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1G10-H8

Détail du produit anti-YDJ1 anticorps

Aucune reactivité croisée: Humain, Souris, Rat (Rattus)

Réactivité croisée (Details): Species reactivity (tested):Yeast.

Purification: Protein G chromatography

Immunogène: Full length protein yeast HSP40 (YDJ1)

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 1/2000. Immunoprecipitation. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: 0.09 % sodium azide in 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur YDJ1

Antigène: YDJ1 (HSP40 / YDJ1 (YDJ1))

Autre désignation: Hsp40 / Ydj1

Sujet: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

ID gène: 850779

UniProt: P39102