CRYAB anticorps

N° du produit ABIN453953

L’anticorps anti-CRYAB Monoclonal Souris est utilisé pour la détection de CRYAB dans des échantillons de Humain et Boeuf (Vache). Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour CRYAB anticorps (ABIN453953)

Antigène: CRYAB (Crystallin, alpha B (CRYAB))

Reactivité: Humain, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CRYAB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 3A10-C9

Détail du produit anti-CRYAB anticorps

Specificité: This antibody detects a band at ~20 kDa (Predicted mol. weight is ~21 kDa) corresponding to Alpha B Crystallin. It Does not cross react with Alpha A Crystallin, Beta-L Crystallin, Beta-H Crystallin, Gamma Crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine.

Attributs du produit: Synonyms: Alpha(B)-crystallin, CRYAB, CRYA2, Heat shock protein beta-5, Renal carcinoma antigenNY-REN-27, HspB5, Rosenthal fiber component

Purification: Protein G Affinity Chromatography

Immunogène: Native alpha B crystallin

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot: 1: 2000.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 50 % Glycerol, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CRYAB

Antigène: CRYAB (Crystallin, alpha B (CRYAB))

Autre désignation: alpha-Crystallin B Chain

Sujet: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alphacrystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).Synonyms: Alpha(B)-crystallin, CRYA2, CRYAB, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

ID gène: 1410

UniProt: P02511