CILP anticorps (Internal Region)

N° du produit ABIN453982

Cet anticorps Chèvre Polyclonal détecte spécifiquement CILP dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Rat, Souris et Chien.

Aperçu rapide pour CILP anticorps (Internal Region) (ABIN453982)

Antigène: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Reactivité: Humain, Rat, Souris, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CILP est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CILP anticorps

Épitope: Internal Region

Specificité: This antibody recognizes CILP.

Réactivité croisée (Details): Species reactivity (expected):Human, Mouse and Canine (Dog).
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-NYRRTDHEDPRVK, from the internal region of the protein sequence according to NP_003604.2.

Information d'application

Indications d'application: Peptide ELISA: Antibody Detection Limit Dilution: 1/128000. Western Blot: 0.1-0.3 μg/mL. Approx 150 kDa band observed in rat spinal cord lysates(calculated MW of 130 kDa according to human NP_003604.2 and of 130 kDa according toRat NP_001101631.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur CILP

Antigène: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Autre désignation: CILP1

Sujet: CILP, Cartilage Intermediate Layer Protein, is specifically expressed in the articular cartilage intermediate layer (it cannot be found in the superficial nor in the deepest layers) and is involved in scaffolding. Overexpression of this protein may lead to impairment of chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Antibodies against CILP are detected in patients with early-stage knee osteoarthritis and defects in this gene may be a cause of susceptibility to lumbar disk disease.Synonyms: CILP-1, Cartilage intermediate layer protein 1, Cartilage intermediate-layer protein

ID gène: 8483

NCBI Accession: NP_003604

UniProt: O75339