GFAP anticorps

N° du produit ABIN457420

Cet anticorps Souris Monoclonal détecte spécifiquement GFAP dans WB, ICC, IHC (p) et IP. Il présente une réactivité envers Humain, Rat et Porc et a été mentionné dans 4+ publications.

Aperçu rapide pour GFAP anticorps (ABIN457420)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: GA-5

Détail du produit anti-GFAP anticorps

Fonction: Anti-GFAP Purified

Specificité: The mouse monoclonal antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.

Réactivité croisée (Details): Human, Porcine, Rat

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Porcine spinal cord

Isotype: IgG1

Information d'application

Indications d'application: Immunocytochemistry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/mL.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein per 1 mL lysate.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-GFAP (ABIN457420)

Joardar, Sen, Das: "Docosahexaenoic acid facilitates cell maturation and beta-adrenergic transmission in astrocytes." dans: Journal of lipid research, Vol. 47, Issue 3, pp. 571-81, (2006) (PubMed).

Guillemin, Wang, Brew: "Quinolinic acid selectively induces apoptosis of human astrocytes: potential role in AIDS dementia complex." dans: Journal of neuroinflammation, Vol. 2, pp. 16, (2005) (PubMed).

Rungger-Brändle, Dosso, Leuenberger: "Glial reactivity, an early feature of diabetic retinopathy." dans: Investigative ophthalmology & visual science, Vol. 41, Issue 7, pp. 1971-80, (2000) (PubMed).

Perng, Cairns, van den IJssel, Prescott, Hutcheson, Quinlan: "Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin." dans: Journal of cell science, Vol. 112 ( Pt 13), pp. 2099-112, (1999) (PubMed).

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Glial fibrillary acidic proteinprovided,GFAP (glial fibrillary acidic protein) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’,s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.,GFAP, ALXDRD

ID gène: 2670

UniProt: P14136