Claudin 1 anticorps (C-Term)

N° du produit ABIN457476

Cet anticorps anti-Claudin 1 Polyclonal Lapin (ABIN457476) détecte spécifiquement Claudin 1 dans WB, IHC, ELISA et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien et Singe.

Aperçu rapide pour Claudin 1 anticorps (C-Term) (ABIN457476)

Antigène: Claudin 1 (CLDN1)

Reactivité: Humain, Souris, Rat, Chien, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Claudin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Claudin 1 anticorps

Épitope: C-Term

Specificité: Recognizes Claudin 1. Species cross-reactivity: Human, Monkey, Mouse, Canine, and Rat.

Purification: Immunoaffinity purified

Immunogène: A synthetic peptide derived from the C-terminus of human Claudin 1.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 2-4 μg/mL for 2 hrs at RT. Positive control: T47D cells, Skin or Breast carcinoma.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.6, 0.2 % BSA, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Claudin 1

Antigène: Claudin 1 (CLDN1)

Autre désignation: CLDN1 / Claudin 1

Sujet: Name/Gene ID: CLDN1
Family: Claudin

Synonyms: CLDN1, Claudin-1, Claudin 1, ILVASC, SEMP1, CLD1

ID gène: 9076

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C