Human Papilloma Virus 18 E2 (HPV-18 E2) (AA 1-83) anticorps

N° du produit ABIN457548

Cet anticorps anti-Human Papilloma Virus 18 E2 Monoclonal Souris (Clone 2E7) (ABIN457548) détecte spécifiquement Human Papilloma Virus 18 E2 dans ELISA, WB et IF. L’anticorps est réactif avec des échantillons de Human Papillomavirus (HPV). Validé indépendamment pour une utilisation en Unfolding Profile.

Aperçu rapide pour Human Papilloma Virus 18 E2 (HPV-18 E2) (AA 1-83) anticorps (ABIN457548)

Antigène: Human Papilloma Virus 18 E2 (HPV-18 E2)

Reactivité: Human Papillomavirus (HPV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Clone: 2E7

Détail du produit

Épitope: AA 1-83

Specificité: Reacts with HPV-18 E2-protein.

Purification: purified with Protein G affinity chromatorgaphy

Immunogène: Recombinant full-length HPV-18 E2-protein purifiedfrom E. coli

Isotype: IgG

Information d'application

Indications d'application: ELISA:1: 2 000, WB: 1: 1000. This antibody does not work in IP. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102723 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102723

Date: 05.11.2018

Antigène: HPV-18

Numéro du lot: A10P2E71-001

Application validée: Unfolding Profile

Contrôle positif: ABIN457548

Conclusion:

Passed. ABIN457548 showed a clear unfolding profile with two detected unfolding events with T_is at 78.4°C and 84.4°C. It is not unusual for antibodies to show more than one unfolding transition, as they are composed of distinct domains that may unfold independently and at different temperatures. This result suggests that the protein is properly folded and functional.

Validation Images

Unfolding profile of ABIN457548. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing two unfolding events. The vertical lines indicate the Tis at 78.4 °C and 84.4 °C.

Protocole

Anticorps primaire: ABIN457548

Full Protocol:

• Dilute ABIN457548 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Concentration: 1 mg/ml

Buffer: PBS pH 7.4, with 0.1%

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: This product is shipped in non-frozen liquid form in ambient conditions

Détail du antigène

Antigène: Human Papilloma Virus 18 E2 (HPV-18 E2)

Classe de substances: Viral Protein

Sujet: Human papillomaviruses(HPV) are small DNA viruses whichinfect epithelia of the skin and mucosa. Over 90 types have been identified andthey mostly cause a variety of benign lesions such as warts and verrucae.However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmedas agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins havecellular transforming and trans-activating activities