LPO anticorps (Biotin)

N° du produit ABIN457588

Cet anticorps Lapin Polyclonal détecte spécifiquement LPO dans WB, ICC, ELISA, DB et IHC (p). Il présente une réactivité avec des échantillons de Boeuf (Vache).

Aperçu rapide pour LPO anticorps (Biotin) (ABIN457588)

Antigène: LPO (Lactoperoxidase (LPO))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LPO est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LPO anticorps (Biotin)

Fonction: Rabbit anti Bovine lactoperoxidase, conjugated with Biotin

Specificité: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine lactoperoxidase.

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of Bovine Serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, but reactivity with lactoperoxidase from Goat and Sheep may be expected.

Attributs du produit: The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user's choice has to be used.

Purification: Purified

Immunogène: Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It sis isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détails sur LPO

Antigène: LPO (Lactoperoxidase (LPO))

Autre désignation: Lactoperoxidase