IgA Secretory Component (Membrane-Bound), (Soluble) anticorps

N° du produit ABIN457835

L’anticorps Souris Monoclonal anti- a été validé pour ELISA, WB, IF et H. Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour IgA Secretory Component (Membrane-Bound), (Soluble) anticorps (ABIN457835)

Antigène: IgA Secretory Component

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Haemagglutination (H)

Clone: NI 194-4 (A89-039)

Détail du produit

Épitope: Membrane-Bound, Soluble

Specificité: The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Attributs du produit: Purified monoclonal mouse antibody to human secretory component, free and bound

Purification: Purified

Immunogène: Highly purified secretory component isolated from human milk.

Isotype: IgG1 kappa

Information d'application

Indications d'application: To identify the presence of free or bound secretory component in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. No foreign proteins added.

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgA Secretory Component

Autre désignation: Secretory Immunoglobulin A (SIgA)

Sujet: The reactivity of the antiserum is restricted to determinants on the secretory component as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).