IgA Secretory Component (Membrane-Bound), (Soluble) anticorps (Biotin)

N° du produit ABIN457848

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans ELISA, WB et IF. Adapté pour Humain.

Aperçu rapide pour IgA Secretory Component (Membrane-Bound), (Soluble) anticorps (Biotin) (ABIN457848)

Antigène: IgA Secretory Component

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Clone: NI 194-4 (A89-39)

Détail du produit

Épitope: Membrane-Bound, Soluble

Specificité: The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Attributs du produit: Biotin-conjugated purified monoclonal mouse antibody to human secretory component, free or bound

Purification: Purified

Immunogène: Highly purified secretory component isolated from human milk.

Isotype: IgG1 kappa

Information d'application

Indications d'application: To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 1.4. No foreign proteins added.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyoph ilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgA Secretory Component

Autre désignation: Secretory Immunoglobulin A (SIgA)

Sujet: The reactivity of the antiserum is restricted to secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining.