Bence-Jones Protein lambda (Chain lambda), (Free & Bound) anticorps (HRP)

N° du produit ABIN457855

Cet anticorps Souris Monoclonal détecte spécifiquement Bence-Jones Protein lambda dans ELISA et WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Bence-Jones Protein lambda (Chain lambda), (Free & Bound) anticorps (HRP) (ABIN457855)

Antigène: Bence-Jones Protein lambda

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB)

Clone: NI 412 and NI 268

Détail du produit

Épitope: Chain lambda, Free & Bound

Specificité: The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Attributs du produit: Horseradish peroxidase conjugated purified monoclonal mouse antibody to human Ig lambda chain, free and bound

Purification: Purified

Immunogène: Highly purified Bence Jones lambda proteins isolated from pooled human urine.

Isotype: IgG1

Information d'application

Indications d'application: To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:50 upwards, in Western blotting from 1:100 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: Bence-Jones Protein lambda

Autre désignation: Bence Jones lambda

Sujet: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains, as tested in direct binding enzyme immunoassay, immuno-blotting, immunoprecipitation and direct immunoperoxidase staining.