IgA Secretory Component anticorps

N° du produit ABIN457970

L’anticorps Chèvre Polyclonal anti- a été validé pour IEP. Il convient pour détecter dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour IgA Secretory Component anticorps (ABIN457970)

Antigène: IgA Secretory Component

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunoelectrophoresis (IEP)

Détail du produit

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homo-logous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Precipitating polyclonal goat antiserum to human secretory component

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion to identify the presence secretory component in human serum or other body fluids and to determine its concentration.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against pooled normal human milk in agar-block immunodiffusion titratio

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiser um by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Référence

Roos Ljungberg, Joshua, Skogh, Eklund, Sköld, Karimi, Nyrén, Svärd, Catrina, Kastbom: "Secretory anti-citrullinated protein antibodies in serum associate with lung involvement in early rheumatoid arthritis." dans: Rheumatology (Oxford, England), Vol. 59, Issue 4, pp. 852-859, (2020) (PubMed).

Roos, Martinsson, Ziegelasch, Sommarin, Svärd, Skogh, Kastbom: "Circulating secretory IgA antibodies against cyclic citrullinated peptides in early rheumatoid arthritis associate with inflammatory activity and smoking." dans: Arthritis research & therapy, Vol. 18, Issue 1, pp. 119, (2017) (PubMed).

Détail du antigène

Antigène: IgA Secretory Component

Autre désignation: Secretory Immunoglobulin A (SIgA)

Sujet: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein