Lactoferrin anticorps (Biotin)

N° du produit ABIN458011

Cet anticorps Chèvre Polyclonal détecte spécifiquement Lactoferrin dans WB, IHC, ELISA et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour Lactoferrin anticorps (Biotin) (ABIN458011)

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactoferrin est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-Lactoferrin anticorps (Biotin)

Specificité: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.

Attributs du produit: Biotin-conjugated IgG fraction of polyclonal goat antiserum to human lactoferrin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized fractions of human serum and lactoferrin-depleted human milk as required, to eliminate antibodies reacting with other human serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the anti-serum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogène: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In immunocytochemical and immunohistochemical techniques for the detection of human lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.5. No foreign proteins added.

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Agent conservateur: Without preservative

Stock: 4 °C

Détails sur Lactoferrin

Antigène: Lactoferrin (LTF) (Lactotransferrin (LTF))

Autre désignation: Lactoferrin

Sujet: Tested in immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma

Pathways: Transition Metal Ion Homeostasis