alpha 2 Antiplasmin anticorps

N° du produit ABIN458040

Cet anticorps anti-alpha 2 Antiplasmin est un anticorps Chèvre Polyclonal détectant alpha 2 Antiplasmin dans ID, IEP et RID. Adapté pour Humain.

Aperçu rapide pour alpha 2 Antiplasmin anticorps (ABIN458040)

Antigène: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha 2 Antiplasmin est non-conjugé

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Détail du produit anti-alpha 2 Antiplasmin anticorps

Specificité: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Precipitating polyclonal goat antiserum to human alpha-2 antiplasmin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Apha-2 antiplasmin is a single-chain glycoprotein with a molecular weight of about 65,000.The normal concentration in human plasma is 60 µ g/ml. Platelets contain significant amounts of the protein, which is released along with platelet factor 4, following thrombin stimulation. It can form 1:1 complexes with circulating plasmin. Alpha-2 antiplasmin is the most important inhibitor of plasmin-mediated fibrinolysis, but also capable of inactivating factor XIIa, kallikrein, factor XIa, thrombin and factor Xa. The importance of the alpha-2 antiplasmin as regulator of the fibrinolytic system was detected by the discovery of a congenital deficiency. Alpha-2 antiplasmin has been isolated from pooled plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). Detection of plasmin-antiplasmin complexes in plasma is evident of plasmin formation and fibrinolysis.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar-block immunodiffusion

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Détails sur alpha 2 Antiplasmin

Antigène: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Autre désignation: alpha-2 Antiplasmin

Sujet: The defined antibody reactivity is restricted to alpha-2 antiplasmin as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones