Hemopexin anticorps

N° du produit ABIN458095

Cet anticorps Lapin Polyclonal détecte spécifiquement Hemopexin dans ELISA, ID, RID, Neph et IEP. Il présente une réactivité envers Humain.

Aperçu rapide pour Hemopexin anticorps (ABIN458095)

Antigène: Hemopexin (HPX)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Hemopexin est non-conjugé

Application: ELISA, Immunodiffusion (ID), Radial Immunodiffusion (RID), Nephelometry (Neph), Immunoelectrophoresis (IEP)

Détail du produit anti-Hemopexin anticorps

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail. In double radial immunodiffusion no reaction has been observed with rat and mouse.

Attributs du produit: Precipitating polyclonal rabbit antiserum to human hemopexin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Hemopexin is a haem-binding beta-globulin present in normal serum at a concentration of 0.8 to 1.0 mg/ml. Its molecular weight is 57,000. The carbohydrate content is 23%. It consists of one polypeptide chain. Purified hemopexin isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence hemopexin in human serum or other body fluids and to determine its concentration. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal human serum in agar-block immunodiffusion titra

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Détails sur Hemopexin

Antigène: Hemopexin (HPX)

Autre désignation: Hemopexin

Sujet: The reactivity of the antiserum is restricted to hemopexin as tested in immunoelectro-phoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified hemopexin

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response