alpha Fetoprotein anticorps (FITC)

N° du produit ABIN458119

Cet anticorps Lapin Polyclonal détecte spécifiquement alpha Fetoprotein dans ELISA, ICC, IHC (fro) et IFA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour alpha Fetoprotein anticorps (FITC) (ABIN458119)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha Fetoprotein est conjugé à/à la FITC

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Détail du produit anti-alpha Fetoprotein anticorps (FITC)

Fonction: Rabbit anti Human alpha-1 foetoprotein, conjugated with FITC

Specificité: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human alphafoetoprotein.

Réactivité croisée (Details): The antiSerum does not cross react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-foetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogène: Purified human alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum, using a proprietary multiple step method. Several foetoproteins have been demonstrated in human foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It cab easily be identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64,000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma. In pooled foetal/newborn serum a concentration of 1 mg/ml has been measured. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détails sur alpha Fetoprotein

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: alpha-Foetoprotein

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process