APOH anticorps

N° du produit ABIN458152

Cet anticorps Lapin Polyclonal détecte spécifiquement APOH dans WB, ELISA, ID et IEP. Il présente une réactivité envers Humain.

Aperçu rapide pour APOH anticorps (ABIN458152)

Antigène: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APOH est non-conjugé

Application: Western Blotting (WB), ELISA, Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Détail du produit anti-APOH anticorps

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Precipitating polyclonal rabbit antiserum to human beta-2 glycoprotein 1 (apolipoprotein H)

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Beta-2 glycoprotein 1 has a molecular weight of 40,000. The mean concentration in adult serum is about 200 µ g/ml. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify the presence of beta-2 glycoprotein 1 or to determine its concentration. The presence of non-precipitating anti-bodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Antibody titre: Measured by quantitative precipitin analysis. The amount of beta2 glycoprotein 1 precipitated by 1 ml a

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Détails sur APOH

Antigène: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Autre désignation: beta-2 Glycoprotein 1 (Apolipoprotein H)

Sujet: In bidimensional immunoelectrophoresis a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma