APOH anticorps

N° du produit ABIN458152

Cet anticorps Lapin Polyclonal détecte spécifiquement APOH dans IEP et RID. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour APOH anticorps (ABIN458152)

Antigène: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp APOH est non-conjugé

Application: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Détail du produit anti-APOH anticorps

Fonction: Rabbit anti Human beta-2 glycoprotein 1

Specificité: Precipitating polyclonal Rabbit antiSerum to Human beta-2 glycoprotein 1 (apolipoprotein H).

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: In bidimensional immunoelectrophoresis a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify the presence of beta-2 glycoprotein 1 or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserumin non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogène: Beta-2 glycoprotein 1 has a molecular weight of 40,000. The mean concentration in adult serum is about 200 mug/ml. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Precipitation assays. In immunoelectrophoresis use 2 μL human plasma or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion use 1 percent antiserum in the gel. Measured by quantitative precipitin analysis. The amount of beta2 glycoprotein 1 precipitated by 1 mL antiserum is approximately 0.2 mg.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détails sur APOH

Antigène: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Autre désignation: beta-2 Glycoprotein 1 (Apolipoprotein H)

UniProt: P02749