Serum anticorps (FITC)

N° du produit ABIN458188

Cet anticorps Porc Polyclonal détecte spécifiquement Serum dans ELISA, IHC (fro), ICC et IFA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Serum anticorps (FITC) (ABIN458188)

Antigène: Serum

Reactivité: Humain

Hôte: Porc

Clonalité: Polyclonal

Conjugué: FITC

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Indirect Immunofluorescence Assay (IFA)

Détail du produit

Fonction: Swine anti Human Serum proteins, conjugated with FITC

Specificité: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Swine antiSerum to Human Serum proteins.

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: In immunoelectrophoresis against normal human serum precipitation can be observed of not less than 15 individual protein components. In direct or indirect fluorescent staining techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogène: Pooled whole human serum of healthy donors and partly purified serum fractions. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: FITC-coupled purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détail du antigène

Antigène: Serum

Autre désignation: Serum Proteins